Aquaporin 1 expression in tissues of canines possessing inherited high K+ erythrocytes
نویسندگان
چکیده
We investigated the expression of aquaporin 1 (AQP1) in tissues from canines with an inherited anomaly that causes their erythrocytes to have high K(+). Northern blot analysis revealed abundant AQP1 expression in lung and kidney, though little expression was found in spleen. Using anti-C-terminus for dog AQP1, abundant expression was shown in kidney, trachea, and eye, but little expression was shown in pancreas and cerebrum, indicating that AQP1 expression in canine tissues is similar to that noted in other mammals.
منابع مشابه
Aquaporin-1 expression in canine peripheral erythrocytes and its relation to cell volume.
To evaluate the relationship between aquaporin-1 (AQP1) expression and the cell volume of red blood cells (RBCs), canine peripheral RBCs were separated according to specific gravity, and expression of the AQP1 protein on the membrane of RBCs was compared using anti-dog AQP1 polypeptide serum. Western blot analysis indicated that there was no significant difference in AQP1 expression between lar...
متن کاملExpression of related proteins and aquaporin genes in grape (Vitis vinifera L.) under salinity sress
Due to worldwide increasing of salinity, the identification of genes conferring tolerance to plants is important. The aim of this study was to investigate salinity effects on the expression of three genes-related to proteins and aquaporin in grape (Vitis vinifera L.). Based on screening study on 18 grape genotypes, H6 and Gharashani that showed lower decrease in water potential, leaf area, leaf...
متن کاملGrowth responses and aquaporin expression in grape genotypes under salinity. Nayer Mohammadkhani*, Reza Heidari, Nasser Abbaspour and Fatemeh Rahmani
The effects of salinity on growth, leaf area and water relations of two grape genotypes (Gharashani and Shirazi) were studied under 2-week salinity (25, 50 and 100 mM NaCl). Growth and fresh weights of all plant parts were significantly (p
متن کاملA Flowcytometry Study of CD55 and CD59 Expression on Erythrocytes in Rheumatoid Arthritis Patients
Background: Inappropriate activation or blockage of the inhibition of complement system could cause tissue damages in autoimmune diseases particularly rheumatoid arthritis (RA). Defect in complement component regulation may cause damages to tissues, on the other hand, or the damaged tissue might affect the unnecessary activation of complement components. Objective: To investigate the expressi...
متن کاملLack of Aquaporin 3 in bovine erythrocyte membranes correlates with low glycerol permeation.
In general, erythrocytes are highly permeable to water, urea and glycerol. However, expression of aquaporin isoforms in erythrocytes appears to be species characteristic. In the present study, human (hRBC) and bovine (bRBC) erythrocytes were chosen for comparative studies due to their significant difference in membrane glycerol permeability. Osmotic water permeability (P(f)) at 23°C was (2.89 ±...
متن کامل